A P19Cl6 GFP reporter line to quantify cardiomyocyte differentiation of stem cells.

A P19Cl6 GFP reporter line to quantify cardiomyocyte differentiation of stem cells.

The clinical application of stem cell therapies is still limited by the ability to produce defined, differentiated cell populations in large numbers in culture. High throughput screens to identify factors which enhance differentiation to particular lineages and promote expansion of precursors in culture are dependent on the development of sensitive and reproducible assays for screening. Here we...

A vector-based system for the differentiation of mouse embryonic stem cells toward germ-line cells

Objective(s):To culture thein vitro mouse embryonic stem cells (mESCs) and to direct their  differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct Stra8-EGFP to select differentiated ES cells that entered meiosis.  Retinoic acid was used to differentiate embryonic stem cells to germ cells. Materials and Methods: A fragment of Stra8 gene pr...

Evaluation of In Vitro Differentiation of Cardiomyocyte-like cells Derived from Human Bone Marrow Mesenchymal Stem Cells

Purpose: To investigate the in vitro differentiation process of cardiomyocyte-like cells derived from human bone marrow mesenchymal stem cells under the influence of 5-azacytidine (5-aza). Materials and Methods: After purification, human bone marrow mesenchymal stem cells were exposed to 5-aza at a concentration of 5 μmol for 5 weeks to induce cardiomyocyte differentiation. To induce differenti...

Comparison of random and aligned PCL nanofibrous electrospun scaffolds on cardiomyocyte differentiation of human adipose-derived stem cells

Objective(s):Cardiomyocytes have small potentials for renovation and proliferation in adult life. The most challenging goal in the field of cardiovascular tissue engineering is the creation of an engineered heart muscle. Tissue engineering with a combination of stem cells and nanofibrous scaffolds has attracted interest with regard to Cardiomyocyte creation applications. Human adipose-derived s...

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells